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Broad Impact Of Oxidative Stress
Broad Impact Of Oxidative Stress
Thalassemia is an iron loading disease therefore is a human model of
iron overload.
Quantitative determination of ortho- and meta-tyrosine as biomarkers
of protein oxidative damage in beta-thalassemia. [Journal Article]
Redox Rep 2007; 12(5):219-28.
Matayatsuk C, Poljak A, Bustamante S, Smythe GA, Kalpravidh RW,
Sirankapracha P, Fucharoen S, Wilairat P
Oxidative stress in thalassemia is caused by secondary iron overload
and stems from blood transfusion and increased iron uptake.
In this study, we hypothesized that levels of o- and m-tyrosine,
products of hydroxyl radical attack on phenylalanine, would be
elevated in beta-thalassemia (intermediate).
This study represents the first report in which specific markers of
protein oxidative damage have been quantified in thalassemia.
We used GC/MS to assay o- and m-tyrosine at the femtomole level using
only a few microliters of plasma.
Levels of both markers were significantly higher in patients with beta-
thalassemia than in controls and were positively correlated with serum
ferritin, malondialdehyde, superoxide dismutase, glutathione
peroxidase and glutathione.
We conclude that o- and m-tyrosine are useful biomarkers of oxidative
damage to proteins in thalassemia (intermediate) and may also be
useful markers in other iron overload diseases. Positive correlations
between o- and m-tyrosine levels and malondialdehyde as well as
antioxidants such as superoxide dismutase, glutathione peroxidase and
glutathione, are indicative of the broad impact of oxidative stress on
blood plasma in thalassemia, with up-regulation of antioxidant
proteins probably reflecting a homeostatic response to these increased
stress levels.
--------------------------------------------------------------------------------
More from this journal
Redox report : communications in free radical research [Redox Rep]
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Thalassemia is an iron loading disease therefore is a human model of
iron overload.
Quantitative determination of ortho- and meta-tyrosine as biomarkers
of protein oxidative damage in beta-thalassemia. [Journal Article]
Redox Rep 2007; 12(5):219-28.
Matayatsuk C, Poljak A, Bustamante S, Smythe GA, Kalpravidh RW,
Sirankapracha P, Fucharoen S, Wilairat P
Oxidative stress in thalassemia is caused by secondary iron overload
and stems from blood transfusion and increased iron uptake.
In this study, we hypothesized that levels of o- and m-tyrosine,
products of hydroxyl radical attack on phenylalanine, would be
elevated in beta-thalassemia (intermediate).
This study represents the first report in which specific markers of
protein oxidative damage have been quantified in thalassemia.
We used GC/MS to assay o- and m-tyrosine at the femtomole level using
only a few microliters of plasma.
Levels of both markers were significantly higher in patients with beta-
thalassemia than in controls and were positively correlated with serum
ferritin, malondialdehyde, superoxide dismutase, glutathione
peroxidase and glutathione.
We conclude that o- and m-tyrosine are useful biomarkers of oxidative
damage to proteins in thalassemia (intermediate) and may also be
useful markers in other iron overload diseases. Positive correlations
between o- and m-tyrosine levels and malondialdehyde as well as
antioxidants such as superoxide dismutase, glutathione peroxidase and
glutathione, are indicative of the broad impact of oxidative stress on
blood plasma in thalassemia, with up-regulation of antioxidant
proteins probably reflecting a homeostatic response to these increased
stress levels.
--------------------------------------------------------------------------------
More from this journal
Redox report : communications in free radical research [Redox Rep]
Who loves ya.
Tom
Jesus Was A Vegetarian!
* tinyurl . com /2r2nkh
Man Is A Herbivore!
* tinyurl . com /a3cc3
DEAD PEOPLE WALKING
* tinyurl . com /zk9fk
Re: Broad Impact Of Oxidative Stress
On Apr 13, 7:47=A0am, ironjustice <teamtan...@hotmail . com > wrote:
Iranian Journal of Pediatrics
Tehran University of Medical Sciences Press
ISSN: 1018-4406
Vol. 17, No. s1, 2007, pp. 73-78
Bioline Code: pe07024
Full paper language: Farsi
Document available free of charge
Iranian Journal of Pediatrics, Vol. 17, No. s1, 2007, pp. 73-78
en Comparison of serum leptin in major =E2-talasemia patients and
normal subjects
Choobineh, H; Dehghani, SJ; Einollahi, N; Kaviani, S; Reiskarami, SR &
Vahedi, S
Abstract
Background: Leptin, is a adipocyte-derived hormone. Exogenous leptin
allows the recovery of the reproductive function. In humans, leptin
correlates positively with body mass index (BMI). The aim of the study
was to investigate the association of leptin with toxic effects of
iron overload.
Methods: In a cross sectional study in 2006, we compared the serum
leptin level of thalasemic patients with normal group. Blood samples
were collected from 219 patients with Cooley's anemia, (119 males, 100
females) and 137 normal subjects (86 males, 51 females). Leptin was
measured by a commercial ELISA kit. Data were analyzed by SPSS
software.
Findings: Mean serum leptin level was 5.33=B1 5.02 ng/ml in thalassaemic
males. It was significantly lower than controls (9.43=B17.8 ng/ml)
(P<0.001). Thalassaemic females had lower leptin levels (12.12=B111.4 ng/
ml) than normal females subjects (14.6=B113.1 ng/ml) (P<0.001).
Furthermore, the physiologically positive BMI/leptin relationship
disappeared in thalassaemic patients.
Conclusions: It seems that the adipocytes of thalassaemic patients are
unable to maintain adequate leptin production. These results suggest
that adipose tissue dysfunction can be considered as one of the
endocrine=ACpathies affecting thalassaemic patients.
Keywords
Beta, -Thalassemia major, Leptin, BMI, Serum iron
Who loves ya.
Tom
Jesus Was A Vegetarian!
* tinyurl . com /2r2nkh
Man Is A Herbivore!
* tinyurl . com /a3cc3
DEAD PEOPLE WALKING
* tinyurl . com /zk9fk
> Thalassemia is an iron loading disease therefore is a human model of
> iron overload.
>
> Quantitative determination of ortho- and meta-tyrosine as biomarkers
> of protein oxidative damage in beta-thalassemia. [Journal Article]
> Redox Rep 2007; 12(5):219-28.
> Matayatsuk C, Poljak A, Bustamante S, Smythe GA, Kalpravidh RW,
> Sirankapracha P, Fucharoen S, Wilairat P
>
> Oxidative stress in thalassemia is caused by secondary iron overload
> and stems from blood transfusion and increased iron uptake.
> In this study, we hypothesized that levels of o- and m-tyrosine,
> products of hydroxyl radical attack on phenylalanine, would be
> elevated in beta-thalassemia (intermediate).
> This study represents the first report in which specific markers of
> protein oxidative damage have been quantified in thalassemia.
> We used GC/MS to assay o- and m-tyrosine at the femtomole level using
> only a few microliters of plasma.
> Levels of both markers were significantly higher in patients with beta-
> thalassemia than in controls and were positively correlated with serum
> ferritin, malondialdehyde, superoxide dismutase, glutathione
> peroxidase and glutathione.
> We conclude that o- and m-tyrosine are useful biomarkers of oxidative
> damage to proteins in thalassemia (intermediate) and may also be
> useful markers in other iron overload diseases. Positive correlations
> between o- and m-tyrosine levels and malondialdehyde as well as
> antioxidants such as superoxide dismutase, glutathione peroxidase and
> glutathione, are indicative of the broad impact of oxidative stress on
> blood plasma in thalassemia, with up-regulation of antioxidant
> proteins probably reflecting a homeostatic response to these increased
> stress levels.
>
> --------------------------------------------------------------------------=
-=AD-----
> More from this journal
> Redox report : communications in free radical research [Redox Rep]
>
> Who loves ya.
> Tom
>
> Jesus Was A Vegetarian! * tinyurl . com /2r2nkh
>
> Man Is A Herbivore! * tinyurl . com /a3cc3
>
> DEAD PEOPLE WALKING * tinyurl . com /zk9fk
On Apr 13, 7:47=A0am, ironjustice <teamtan...@hotmail . com > wrote:
Iranian Journal of Pediatrics
Tehran University of Medical Sciences Press
ISSN: 1018-4406
Vol. 17, No. s1, 2007, pp. 73-78
Bioline Code: pe07024
Full paper language: Farsi
Document available free of charge
Iranian Journal of Pediatrics, Vol. 17, No. s1, 2007, pp. 73-78
en Comparison of serum leptin in major =E2-talasemia patients and
normal subjects
Choobineh, H; Dehghani, SJ; Einollahi, N; Kaviani, S; Reiskarami, SR &
Vahedi, S
Abstract
Background: Leptin, is a adipocyte-derived hormone. Exogenous leptin
allows the recovery of the reproductive function. In humans, leptin
correlates positively with body mass index (BMI). The aim of the study
was to investigate the association of leptin with toxic effects of
iron overload.
Methods: In a cross sectional study in 2006, we compared the serum
leptin level of thalasemic patients with normal group. Blood samples
were collected from 219 patients with Cooley's anemia, (119 males, 100
females) and 137 normal subjects (86 males, 51 females). Leptin was
measured by a commercial ELISA kit. Data were analyzed by SPSS
software.
Findings: Mean serum leptin level was 5.33=B1 5.02 ng/ml in thalassaemic
males. It was significantly lower than controls (9.43=B17.8 ng/ml)
(P<0.001). Thalassaemic females had lower leptin levels (12.12=B111.4 ng/
ml) than normal females subjects (14.6=B113.1 ng/ml) (P<0.001).
Furthermore, the physiologically positive BMI/leptin relationship
disappeared in thalassaemic patients.
Conclusions: It seems that the adipocytes of thalassaemic patients are
unable to maintain adequate leptin production. These results suggest
that adipose tissue dysfunction can be considered as one of the
endocrine=ACpathies affecting thalassaemic patients.
Keywords
Beta, -Thalassemia major, Leptin, BMI, Serum iron
Who loves ya.
Tom
Jesus Was A Vegetarian!
* tinyurl . com /2r2nkh
Man Is A Herbivore!
* tinyurl . com /a3cc3
DEAD PEOPLE WALKING
* tinyurl . com /zk9fk
> Thalassemia is an iron loading disease therefore is a human model of
> iron overload.
>
> Quantitative determination of ortho- and meta-tyrosine as biomarkers
> of protein oxidative damage in beta-thalassemia. [Journal Article]
> Redox Rep 2007; 12(5):219-28.
> Matayatsuk C, Poljak A, Bustamante S, Smythe GA, Kalpravidh RW,
> Sirankapracha P, Fucharoen S, Wilairat P
>
> Oxidative stress in thalassemia is caused by secondary iron overload
> and stems from blood transfusion and increased iron uptake.
> In this study, we hypothesized that levels of o- and m-tyrosine,
> products of hydroxyl radical attack on phenylalanine, would be
> elevated in beta-thalassemia (intermediate).
> This study represents the first report in which specific markers of
> protein oxidative damage have been quantified in thalassemia.
> We used GC/MS to assay o- and m-tyrosine at the femtomole level using
> only a few microliters of plasma.
> Levels of both markers were significantly higher in patients with beta-
> thalassemia than in controls and were positively correlated with serum
> ferritin, malondialdehyde, superoxide dismutase, glutathione
> peroxidase and glutathione.
> We conclude that o- and m-tyrosine are useful biomarkers of oxidative
> damage to proteins in thalassemia (intermediate) and may also be
> useful markers in other iron overload diseases. Positive correlations
> between o- and m-tyrosine levels and malondialdehyde as well as
> antioxidants such as superoxide dismutase, glutathione peroxidase and
> glutathione, are indicative of the broad impact of oxidative stress on
> blood plasma in thalassemia, with up-regulation of antioxidant
> proteins probably reflecting a homeostatic response to these increased
> stress levels.
>
> --------------------------------------------------------------------------=
-=AD-----
> More from this journal
> Redox report : communications in free radical research [Redox Rep]
>
> Who loves ya.
> Tom
>
> Jesus Was A Vegetarian! * tinyurl . com /2r2nkh
>
> Man Is A Herbivore! * tinyurl . com /a3cc3
>
> DEAD PEOPLE WALKING * tinyurl . com /zk9fk
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