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Infection and Lactoferrin
Infection and Lactoferrin
Lacasse P, Lauzon K, Diarra MS, Petitclerc D
Utilization of lactoferrin to fight antibiotic resistant mammary gland
pathogens. [JOURNAL ARTICLE]
J Anim Sci 2007 Jun 12.
The widespread use of antibiotics has lead to the increased presence
of pathogens that are less susceptible to their antibacterial effect.
Lactoferrin (Lf) is naturally produced by the mammary gland.
Lactoferrin is the main whey protein in human milk and is also present
in cow's milk but at a much lower concentration than in human milk.
This protein appears to have many biological functions, including
antibacterial and anti-inflammatory activities.
The best known effect of Lf is to bind iron that is essential for
bacterial growth.
However, the cationic nature of this protein also appears important
for the antimicrobial activity of this protein.
Lactoferrin has a weak antibacterial effect when used alone but,
interestingly, Lf appears much more effective when used at low
concentration in combination with several antibiotics.
The most striking observation is that Lf increases the inhibitory
activity of penicillin up to four fold in most penicillin-susceptible
Staphylococcus aureus strains, whereas this increase was 4 to 16 fold
in penicillin-resistant strains.
Indeed, Lf reduces beta-lactamase activity in S. aureus strains
producing this enzyme. Transcription of beta-lactamase gene is
dramatically repressed in the presence of Lf.
We evaluated the efficacy of intramammary treatments containing
penicillin G and(or) bovine Lf (bLf) to cure chronic mastitis caused
by a clinical isolate of S. aureus highly resistant to beta-lactam
antibiotics.
In a first trial, mastitis was induced in lactating cows by injecting
a low dose of S. aureus through the teat canal of all quarters.
Bacterial cure rate was null for control quarters, 11.1% for bLf, 9.1%
for penicillin and 45.5% for the combination.
A second trial was undertaken to investigate the effect of an extended
therapy on chronic mastitis acquired in a previous lactation.
Quarters were treated with 100,000 IU of penicillin G with or without
250 mg bLf for 7 d.
Bacterial cure rate was higher for the bLf + penicillin combination
(33.3%) comparatively to penicillin alone (12.5%).
In conclusion, bLf added to penicillin is an effective combination for
the treatment of stable S. aureus infections resistant to beta-lactam
antibiotics.
J Anim Sci
--------------------------------------------------------------------------------
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Tom
Jesus Was A Vegetarian!
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Lacasse P, Lauzon K, Diarra MS, Petitclerc D
Utilization of lactoferrin to fight antibiotic resistant mammary gland
pathogens. [JOURNAL ARTICLE]
J Anim Sci 2007 Jun 12.
The widespread use of antibiotics has lead to the increased presence
of pathogens that are less susceptible to their antibacterial effect.
Lactoferrin (Lf) is naturally produced by the mammary gland.
Lactoferrin is the main whey protein in human milk and is also present
in cow's milk but at a much lower concentration than in human milk.
This protein appears to have many biological functions, including
antibacterial and anti-inflammatory activities.
The best known effect of Lf is to bind iron that is essential for
bacterial growth.
However, the cationic nature of this protein also appears important
for the antimicrobial activity of this protein.
Lactoferrin has a weak antibacterial effect when used alone but,
interestingly, Lf appears much more effective when used at low
concentration in combination with several antibiotics.
The most striking observation is that Lf increases the inhibitory
activity of penicillin up to four fold in most penicillin-susceptible
Staphylococcus aureus strains, whereas this increase was 4 to 16 fold
in penicillin-resistant strains.
Indeed, Lf reduces beta-lactamase activity in S. aureus strains
producing this enzyme. Transcription of beta-lactamase gene is
dramatically repressed in the presence of Lf.
We evaluated the efficacy of intramammary treatments containing
penicillin G and(or) bovine Lf (bLf) to cure chronic mastitis caused
by a clinical isolate of S. aureus highly resistant to beta-lactam
antibiotics.
In a first trial, mastitis was induced in lactating cows by injecting
a low dose of S. aureus through the teat canal of all quarters.
Bacterial cure rate was null for control quarters, 11.1% for bLf, 9.1%
for penicillin and 45.5% for the combination.
A second trial was undertaken to investigate the effect of an extended
therapy on chronic mastitis acquired in a previous lactation.
Quarters were treated with 100,000 IU of penicillin G with or without
250 mg bLf for 7 d.
Bacterial cure rate was higher for the bLf + penicillin combination
(33.3%) comparatively to penicillin alone (12.5%).
In conclusion, bLf added to penicillin is an effective combination for
the treatment of stable S. aureus infections resistant to beta-lactam
antibiotics.
J Anim Sci
--------------------------------------------------------------------------------
Who loves ya.
Tom
Jesus Was A Vegetarian!
* tinyurl . com /2r2nkh
Man Is A Herbivore!
* tinyurl . com /a3cc3
DEAD PEOPLE WALKING
* tinyurl . com /zk9fk
