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ECGC highly synergistic with ibuprofen against prostate cancer
ECGC highly synergistic with ibuprofen against prostate cancer
Key sentence from abstract: "EGCG + ibuprofen treatment resulted in 90%
growth inhibition, while ibuprofen or EGCG alone reduced cell numbers by 25%
and 20%, respectively." But don't use NAC with either.
* w w w .ncbi.nlm.nih.gov/pubmed/18225555?ordinalpos=14&itool=EntrezSystem
2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum
Anticancer Res. 2007 Nov-Dec;27(6B):3947-56.Links
Synergistic cell death by EGCG and ibuprofen in DU-145 prostate cancer cell
line.
Kim MH, Chung J.
Department of Molecular Biology and Immunology, University of North Texas
Health Science Center, Fort Worth, Texas 76107, USA. mkim@hsc.unt.edu
BACKGROUND: One of the green tea components epigallocatechin-3-gallate
(EGCG) significantly prevented the growth of prostate cancer cells. In this
study, synergistic effect of EGCG and ibuprofen (EGCG+ibuprofen) was
investigated to determine their anti-proliferative and pro-apoptotic action
in DU-145 prostate cancer cells. MATERIALS AND METHODS: Cell death analysis,
immunoblotting, RT-PCR analysis, and caspase activity assay were used.
RESULTS: EGCG+ibuprofen treatment resulted in 90% growth inhibition, while
ibuprofen or EGCG alone reduced cell numbers by 25% and 20%, respectively.
EGCG+ibuprofen induced MAPK activation, caspase activation and the
inhibition of Bfl-1 expression, all of which were blocked by the
antioxidant, N-acetyl-L-cysteine (NAC). Moreover, addition of ceramide
rescued the NAC-inhibited MAPK activation and pretreatment with the ceramide
synthase inhibitor, fumonisin B1, reduced cell death. CONCLUSION: Our
results suggest that in DU-145 prostate cancer cells: (i) EGCG+ibuprofen
treatment has a synergistic effect on apoptosis, and (ii) oxidative stress,
directly or indirectly via ceramide synthesis mediates pro-apoptotic
signaling.
Key sentence from abstract: "EGCG + ibuprofen treatment resulted in 90%
growth inhibition, while ibuprofen or EGCG alone reduced cell numbers by 25%
and 20%, respectively." But don't use NAC with either.
* w w w .ncbi.nlm.nih.gov/pubmed/18225555?ordinalpos=14&itool=EntrezSystem
2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum
Anticancer Res. 2007 Nov-Dec;27(6B):3947-56.Links
Synergistic cell death by EGCG and ibuprofen in DU-145 prostate cancer cell
line.
Kim MH, Chung J.
Department of Molecular Biology and Immunology, University of North Texas
Health Science Center, Fort Worth, Texas 76107, USA. mkim@hsc.unt.edu
BACKGROUND: One of the green tea components epigallocatechin-3-gallate
(EGCG) significantly prevented the growth of prostate cancer cells. In this
study, synergistic effect of EGCG and ibuprofen (EGCG+ibuprofen) was
investigated to determine their anti-proliferative and pro-apoptotic action
in DU-145 prostate cancer cells. MATERIALS AND METHODS: Cell death analysis,
immunoblotting, RT-PCR analysis, and caspase activity assay were used.
RESULTS: EGCG+ibuprofen treatment resulted in 90% growth inhibition, while
ibuprofen or EGCG alone reduced cell numbers by 25% and 20%, respectively.
EGCG+ibuprofen induced MAPK activation, caspase activation and the
inhibition of Bfl-1 expression, all of which were blocked by the
antioxidant, N-acetyl-L-cysteine (NAC). Moreover, addition of ceramide
rescued the NAC-inhibited MAPK activation and pretreatment with the ceramide
synthase inhibitor, fumonisin B1, reduced cell death. CONCLUSION: Our
results suggest that in DU-145 prostate cancer cells: (i) EGCG+ibuprofen
treatment has a synergistic effect on apoptosis, and (ii) oxidative stress,
directly or indirectly via ceramide synthesis mediates pro-apoptotic
signaling.
