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Targeting iron in cancer
Targeting iron in cancer
Downregulation of transferrin receptor surface expression by
intracellular antibody.
Peng JL, Wu S, Zhao XP, Wang M, Li WH, Shen X, Liu J, Lei P, Zhu HF,
Shen GX
Biochem Biophys Res Commun. 2007 Mar 23; 354(4): 864-71
To deplete cellular iron uptake, and consequently inhibit the
proliferation of tumor cells, we attempt to block surface expression
of transferrin receptor (TfR) by intracellular antibody technology. We
constructed two expression plasmids (scFv-HAK and scFv-HA) coding for
intracellular single-chain antibody against TfR with or without
endoplasmic reticulum (ER) retention signal, respectively. Then they
were transfected tumor cells MCF-7 by liposome. Applying RT-PCR,
Western blotting, immunofluorescence microscopy and immunoelectron
microscope experiments, we insure that scFv-HAK intrabody was
successfully expressed and retained in ER contrasted to the secreted
expression of scFv-HA. Flow cytometric analysis confirmed that the TfR
surface expression was markedly decreased approximately 83.4+/-2.5% in
scFv-HAK transfected cells, while there was not significantly decrease
in scFv-HA transfected cells. Further cell growth and apoptosis
characteristics were evaluated by cell cycle analysis, nuclei staining
and MTT assay. Results indicated that expression of scFv-HAK can
dramatically induce cell cycle G1 phase arrest and apoptosis of tumor
cells, and consequently significantly suppress proliferation of tumor
cells compared with other control groups. For the first time this
study demonstrates the potential usage of anti-TfR scFv-intrabody as a
growth inhibitor of TfR overexpressing tumors.
Who loves ya.
Tom
Jesus Was A Vegetarian!
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DEAD PEOPLE WALKING
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Downregulation of transferrin receptor surface expression by
intracellular antibody.
Peng JL, Wu S, Zhao XP, Wang M, Li WH, Shen X, Liu J, Lei P, Zhu HF,
Shen GX
Biochem Biophys Res Commun. 2007 Mar 23; 354(4): 864-71
To deplete cellular iron uptake, and consequently inhibit the
proliferation of tumor cells, we attempt to block surface expression
of transferrin receptor (TfR) by intracellular antibody technology. We
constructed two expression plasmids (scFv-HAK and scFv-HA) coding for
intracellular single-chain antibody against TfR with or without
endoplasmic reticulum (ER) retention signal, respectively. Then they
were transfected tumor cells MCF-7 by liposome. Applying RT-PCR,
Western blotting, immunofluorescence microscopy and immunoelectron
microscope experiments, we insure that scFv-HAK intrabody was
successfully expressed and retained in ER contrasted to the secreted
expression of scFv-HA. Flow cytometric analysis confirmed that the TfR
surface expression was markedly decreased approximately 83.4+/-2.5% in
scFv-HAK transfected cells, while there was not significantly decrease
in scFv-HA transfected cells. Further cell growth and apoptosis
characteristics were evaluated by cell cycle analysis, nuclei staining
and MTT assay. Results indicated that expression of scFv-HAK can
dramatically induce cell cycle G1 phase arrest and apoptosis of tumor
cells, and consequently significantly suppress proliferation of tumor
cells compared with other control groups. For the first time this
study demonstrates the potential usage of anti-TfR scFv-intrabody as a
growth inhibitor of TfR overexpressing tumors.
Who loves ya.
Tom
Jesus Was A Vegetarian!
* jesuswasavegetarian.7h . com
Man Is A Herbivore!
* tinyurl . com /a3cc3
DEAD PEOPLE WALKING
* tinyurl . com /zk9fk
