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calci-chew
calci-chew
Hi Members, I am new to the group.Recently found out I have osteoporosis,
age 52 female, menopause at age 40 .
I was prescribed actonel and calci chew but am trying to find homeopathic
alternatives as I don't like the sound of either one.
Is anyone out there taking a calcium+D3 supplement that does not have
aspartame in it or other sugar substitutes? I had started taking the calci
chew but the flyer mentions aspartame as ingredient.
Thanks, Pam
Hi Members, I am new to the group.Recently found out I have osteoporosis,
age 52 female, menopause at age 40 .
I was prescribed actonel and calci chew but am trying to find homeopathic
alternatives as I don't like the sound of either one.
Is anyone out there taking a calcium+D3 supplement that does not have
aspartame in it or other sugar substitutes? I had started taking the calci
chew but the flyer mentions aspartame as ingredient.
Thanks, Pam
Re: calci-chew
On Tue, 25 Dec 2007 15:24:28 +0100, "perkens" <perkens@home.nl> wrote:
>Hi Members, I am new to the group.Recently found out I have osteoporosis,
>age 52 female, menopause at age 40 .
>
>I was prescribed actonel and calci chew but am trying to find homeopathic
>alternatives as I don't like the sound of either one.
>Is anyone out there taking a calcium+D3 supplement that does not have
>aspartame in it or other sugar substitutes? I had started taking the calci
>chew but the flyer mentions aspartame as ingredient.
>
>Thanks, Pam
>
I take Inno-Vite, which is calcium, phosphorus,Vit D3 and Zinc in a
base of Sunflower oil, eviening primrose oil and canola oil. Has no
yeast, sugar, starch, wheat,corn, milk, eggs, colours, flavours or
preservatives.
My grocery store carries it in the pharmacy area.
Mari
On Tue, 25 Dec 2007 15:24:28 +0100, "perkens" <perkens@home.nl> wrote:
>Hi Members, I am new to the group.Recently found out I have osteoporosis,
>age 52 female, menopause at age 40 .
>
>I was prescribed actonel and calci chew but am trying to find homeopathic
>alternatives as I don't like the sound of either one.
>Is anyone out there taking a calcium+D3 supplement that does not have
>aspartame in it or other sugar substitutes? I had started taking the calci
>chew but the flyer mentions aspartame as ingredient.
>
>Thanks, Pam
>
I take Inno-Vite, which is calcium, phosphorus,Vit D3 and Zinc in a
base of Sunflower oil, eviening primrose oil and canola oil. Has no
yeast, sugar, starch, wheat,corn, milk, eggs, colours, flavours or
preservatives.
My grocery store carries it in the pharmacy area.
Mari
Re: calci-chew
Thank you for your answers. I will be taking a bone protect mix with D3, K1,
C, calcium citrate, magnesium, copper, mangenese, selenium, zink and
borium -
plus an extra D3 supplement, silica, a soy supplement and urticalcin (from
stinging nettles, which is meant to help proper uptake of calcium) all
prescribed by homeopathic practitioners. Will get a new Dexa scan in Sept or
October next year. Also see a physical therapist in a gym-like setting for
exercises set up for my osteoporosis and also use power plate w/therapist.
Walking every day, hand weights.
Thanks and good luck to all of us!
Pam (American living in The Netherlands)
<mari@net.ac> schreef in bericht
news:7p42n3ph2s41t7jjocnhpd5lj82hgip4i6@4ax . com ...
> On Tue, 25 Dec 2007 15:24:28 +0100, "perkens" <perkens@home.nl> wrote:
>
>>Hi Members, I am new to the group.Recently found out I have osteoporosis,
>>age 52 female, menopause at age 40 .
>>
>>I was prescribed actonel and calci chew but am trying to find homeopathic
>>alternatives as I don't like the sound of either one.
>>Is anyone out there taking a calcium+D3 supplement that does not have
>>aspartame in it or other sugar substitutes? I had started taking the calci
>>chew but the flyer mentions aspartame as ingredient.
>>
>>Thanks, Pam
>>
>
> I take Inno-Vite, which is calcium, phosphorus,Vit D3 and Zinc in a
> base of Sunflower oil, eviening primrose oil and canola oil. Has no
> yeast, sugar, starch, wheat,corn, milk, eggs, colours, flavours or
> preservatives.
>
> My grocery store carries it in the pharmacy area.
>
> Mari
Thank you for your answers. I will be taking a bone protect mix with D3, K1,
C, calcium citrate, magnesium, copper, mangenese, selenium, zink and
borium -
plus an extra D3 supplement, silica, a soy supplement and urticalcin (from
stinging nettles, which is meant to help proper uptake of calcium) all
prescribed by homeopathic practitioners. Will get a new Dexa scan in Sept or
October next year. Also see a physical therapist in a gym-like setting for
exercises set up for my osteoporosis and also use power plate w/therapist.
Walking every day, hand weights.
Thanks and good luck to all of us!
Pam (American living in The Netherlands)
<mari@net.ac> schreef in bericht
news:7p42n3ph2s41t7jjocnhpd5lj82hgip4i6@4ax . com ...
> On Tue, 25 Dec 2007 15:24:28 +0100, "perkens" <perkens@home.nl> wrote:
>
>>Hi Members, I am new to the group.Recently found out I have osteoporosis,
>>age 52 female, menopause at age 40 .
>>
>>I was prescribed actonel and calci chew but am trying to find homeopathic
>>alternatives as I don't like the sound of either one.
>>Is anyone out there taking a calcium+D3 supplement that does not have
>>aspartame in it or other sugar substitutes? I had started taking the calci
>>chew but the flyer mentions aspartame as ingredient.
>>
>>Thanks, Pam
>>
>
> I take Inno-Vite, which is calcium, phosphorus,Vit D3 and Zinc in a
> base of Sunflower oil, eviening primrose oil and canola oil. Has no
> yeast, sugar, starch, wheat,corn, milk, eggs, colours, flavours or
> preservatives.
>
> My grocery store carries it in the pharmacy area.
>
> Mari
Re: calci-chew
perkens wrote:
> Hi Members, I am new to the group.Recently found out I have osteoporosis,
> age 52 female, menopause at age 40 .
>
> I was prescribed actonel and calci chew but am trying to find homeopathic
> alternatives as I don't like the sound of either one.
> Is anyone out there taking a calcium+D3 supplement that does not have
> aspartame in it or other sugar substitutes? I had started taking the calci
> chew but the flyer mentions aspartame as ingredient.
>
> Thanks, Pam
>
>
Suggest that you carefully monitor yourself with a Dexa-Scan within 6-12
months after you try something homeopathic to see if it's stable or
getting better or worse. You don't want to end up with a fracture in a
place like a vertebra or a hip ... they are hell to fix.
Larry
perkens wrote:
> Hi Members, I am new to the group.Recently found out I have osteoporosis,
> age 52 female, menopause at age 40 .
>
> I was prescribed actonel and calci chew but am trying to find homeopathic
> alternatives as I don't like the sound of either one.
> Is anyone out there taking a calcium+D3 supplement that does not have
> aspartame in it or other sugar substitutes? I had started taking the calci
> chew but the flyer mentions aspartame as ingredient.
>
> Thanks, Pam
>
>
Suggest that you carefully monitor yourself with a Dexa-Scan within 6-12
months after you try something homeopathic to see if it's stable or
getting better or worse. You don't want to end up with a fracture in a
place like a vertebra or a hip ... they are hell to fix.
Larry
Re: calci-chew
>
> Suggest that you carefully monitor yourself with a Dexa-Scan within 6-12
> months after you try something homeopathic to see if it's stable or
> getting better or worse. You don't want to end up with a fracture in a
> place like a vertebra or a hip ... they are hell to fix.
>
> Larry
I'm new to this group.
This might be true in other cities but during Osteoporosis Month the
Orthopaedic Hospital in Los Angeles gives dexa scans at a reduced
price. The initial test is $10 more than subsequent tests. I think the
tests are currently $69. Actually I really think you can call any time
of year and ask if they are giving the Osteoporosis Month special.
Carlan
>
> Suggest that you carefully monitor yourself with a Dexa-Scan within 6-12
> months after you try something homeopathic to see if it's stable or
> getting better or worse. You don't want to end up with a fracture in a
> place like a vertebra or a hip ... they are hell to fix.
>
> Larry
I'm new to this group.
This might be true in other cities but during Osteoporosis Month the
Orthopaedic Hospital in Los Angeles gives dexa scans at a reduced
price. The initial test is $10 more than subsequent tests. I think the
tests are currently $69. Actually I really think you can call any time
of year and ask if they are giving the Osteoporosis Month special.
Carlan
Re: calci-chew
On Dec 25, 6:24 am, "perkens" <perk...@home.nl> wrote:
> Hi Members, I am new to the group.Recently found out I have osteoporosis,
> age 52 female, menopause at age 40 .
>
> I was prescribed actonel and calci chew but am trying to find homeopathic
> alternatives as I don't like the sound of either one.
> Is anyone out there taking a calcium+D3 supplement that does not have
> aspartame in it or other sugar substitutes? I had started taking the calci=
> chew but the flyer mentions aspartame as ingredient.
>
> Thanks, Pam
High dose vitamins, minerals, nutrients, and herbs are not
homeopathy the way I understand it. Perhaps the word takes
in naturopathy practice were you are in popular usage?
The use of vitamin K and D in liberal dose is likely a good idea
as most people suffer either deficiency or insufficiency much
of the time.
I suggest you add rutin to your nutrient regimen.
Inflammation tends to speed bone breakdown.
More fruit and vegetables to keep the potassium level
up is also good in this context.
I am of the opinion hormones should have a place in the
treatment of osteo but currently they are in disfavor
due to the flawed hormone regimens marketed to and used
by the mainstream Doctors.
Don't reject the conventional meds, if you need them.
You can use them and take the nutrients as well.
On Dec 25, 6:24 am, "perkens" <perk...@home.nl> wrote:
> Hi Members, I am new to the group.Recently found out I have osteoporosis,
> age 52 female, menopause at age 40 .
>
> I was prescribed actonel and calci chew but am trying to find homeopathic
> alternatives as I don't like the sound of either one.
> Is anyone out there taking a calcium+D3 supplement that does not have
> aspartame in it or other sugar substitutes? I had started taking the calci=
> chew but the flyer mentions aspartame as ingredient.
>
> Thanks, Pam
High dose vitamins, minerals, nutrients, and herbs are not
homeopathy the way I understand it. Perhaps the word takes
in naturopathy practice were you are in popular usage?
The use of vitamin K and D in liberal dose is likely a good idea
as most people suffer either deficiency or insufficiency much
of the time.
I suggest you add rutin to your nutrient regimen.
Inflammation tends to speed bone breakdown.
More fruit and vegetables to keep the potassium level
up is also good in this context.
I am of the opinion hormones should have a place in the
treatment of osteo but currently they are in disfavor
due to the flawed hormone regimens marketed to and used
by the mainstream Doctors.
Don't reject the conventional meds, if you need them.
You can use them and take the nutrients as well.
Re: calci-chew
Hi, yes, homeopathy is used rather generically to include naturopathy. Sorry
for the confusion on that score. The doctor I am seeing is schooled and
licensed in both traditional and naturopathic medicine. Thanks for the tip
on rutin, I had never heard of it.
We try to keep our diet alkaline as acidic body environment also can cause
resorption of the bones. Also Take flax seed oil and ground flax seed every
day in kwark or bio yogurt for essential fatty acid omega 3. Don't eat meat,
eat plenty of veg and fruit and only whole wheat meal/flour. No sugars. I
have now switched to decaf coffee.
Appreciate all your input.
Pam
<trigonometry1972@gmail . com > schreef in bericht
news:5139449d-9866-490a-b1c6-5aea36390321@i29g2000prf.googlegroups . com ...
On Dec 25, 6:24 am, "perkens" <perk...@home.nl> wrote:
> Hi Members, I am new to the group.Recently found out I have osteoporosis,
> age 52 female, menopause at age 40 .
>
> I was prescribed actonel and calci chew but am trying to find homeopathic
> alternatives as I don't like the sound of either one.
> Is anyone out there taking a calcium+D3 supplement that does not have
> aspartame in it or other sugar substitutes? I had started taking the calci
> chew but the flyer mentions aspartame as ingredient.
>
> Thanks, Pam
High dose vitamins, minerals, nutrients, and herbs are not
homeopathy the way I understand it. Perhaps the word takes
in naturopathy practice were you are in popular usage?
The use of vitamin K and D in liberal dose is likely a good idea
as most people suffer either deficiency or insufficiency much
of the time.
I suggest you add rutin to your nutrient regimen.
Inflammation tends to speed bone breakdown.
More fruit and vegetables to keep the potassium level
up is also good in this context.
I am of the opinion hormones should have a place in the
treatment of osteo but currently they are in disfavor
due to the flawed hormone regimens marketed to and used
by the mainstream Doctors.
Don't reject the conventional meds, if you need them.
You can use them and take the nutrients as well.
Hi, yes, homeopathy is used rather generically to include naturopathy. Sorry
for the confusion on that score. The doctor I am seeing is schooled and
licensed in both traditional and naturopathic medicine. Thanks for the tip
on rutin, I had never heard of it.
We try to keep our diet alkaline as acidic body environment also can cause
resorption of the bones. Also Take flax seed oil and ground flax seed every
day in kwark or bio yogurt for essential fatty acid omega 3. Don't eat meat,
eat plenty of veg and fruit and only whole wheat meal/flour. No sugars. I
have now switched to decaf coffee.
Appreciate all your input.
Pam
<trigonometry1972@gmail . com > schreef in bericht
news:5139449d-9866-490a-b1c6-5aea36390321@i29g2000prf.googlegroups . com ...
On Dec 25, 6:24 am, "perkens" <perk...@home.nl> wrote:
> Hi Members, I am new to the group.Recently found out I have osteoporosis,
> age 52 female, menopause at age 40 .
>
> I was prescribed actonel and calci chew but am trying to find homeopathic
> alternatives as I don't like the sound of either one.
> Is anyone out there taking a calcium+D3 supplement that does not have
> aspartame in it or other sugar substitutes? I had started taking the calci
> chew but the flyer mentions aspartame as ingredient.
>
> Thanks, Pam
High dose vitamins, minerals, nutrients, and herbs are not
homeopathy the way I understand it. Perhaps the word takes
in naturopathy practice were you are in popular usage?
The use of vitamin K and D in liberal dose is likely a good idea
as most people suffer either deficiency or insufficiency much
of the time.
I suggest you add rutin to your nutrient regimen.
Inflammation tends to speed bone breakdown.
More fruit and vegetables to keep the potassium level
up is also good in this context.
I am of the opinion hormones should have a place in the
treatment of osteo but currently they are in disfavor
due to the flawed hormone regimens marketed to and used
by the mainstream Doctors.
Don't reject the conventional meds, if you need them.
You can use them and take the nutrients as well.
Re: calci-chew
A collection of abstracts that suggest
rutin, quercetin, and other flavonoids help
preserve bone.
Here in the States it is possible to buy both
bulk quercetin and rutin powders as well as
tablets. Quercetin powder is very light
and is a little hard to mix into water.
Some foods are pretty good sources.
The USDA has some downloads on
food sources for flavonoids and related
substances.
==========================
===
Zhong Yao Cai. 2006 May;29(5):467-70.
[Comparative study on effects of rutin and quercetin
on metabolism in osteoblast
cells]
[Article in Chinese]
Yang YJ, Yang ZL, Wang DC, Xiao XC, Li P.
Department of Traditional Chinese Medicine,
China Pharmaceutical University,
Nanjing 210038, China.
yangyajunl@163 . com
OBJECTIVE:
To investigate the mechanism of rutin and its aglycone
(quercetin) in preventing and treating osteoporosis and
their effects on stimulating bone
formation were studied comparatively in osteoblast cells.
METHODS:
The effects of rutin and quercetin on the proliferation,
differentiation and mineralization were studied respectively
by using tetrazolium (MTT), alkaline phosphatase (ALP) and
mineralized nodules assays in newborn rat calvarial
osteoblast (ROB) cells.
RESULTS:
Quercetin showed significant stimulatory effects on
proliferation and mineralization in ROB cells, so it could
promote bone formation. However, rutin could not improve
stimulation of bone formation because it increased
proliferation and inhibited differentation and had
no remarkable influence on ROB cells in vitro.
CONCLUSION:
Rutin showed less activity than quercetin on bone
formation in ROB cells in vitro. Therefore the
mechanism that some foods and traditional Chinese medicine
containing rutin can prevent and treat osteoporosis
is that its metaboite in vivo can promote bone formation
in ROB cells.
PMID: 16981462
==========================
=====================
Eur J Pharmacol. 2004 Feb 6;485(1-3):89-96.
Quercetin, a flavonoid, inhibits the proliferation,
differentiation, and mineralization of osteoblasts in vitro.
Notoya M, Tsukamoto Y, Nishimura H, Woo JT, Nagai K,
Lee IS, Hagiwara H.
Department of Biological Sciences,
Tokyo Institute of Technology, 4259
Nagatsuta-cho, Midori-ku,
Yokohama 226-8501, Japan.
It is possible that the flavonoids that are found in
many foods might have a protective effect against osteoclastic
activity. However, little information is available about
the effects of flavonoids on osteoblastogenesis.
Therefore, we investigated the effects of quercetin,
a flavonoid, on the metabolism of rat
calvarial osteoblast-like cells (ROB cells) in culture.
The proliferation of cells was markedly inhibited upon
exposure of cells to quercetin at 5 x 10(-6) to
1 x 10(-5) M. Quercetin at 1 x 10(-5) M did not
induce apoptosis in ROB cells but arrested cells
at the G1 phase of the cell cycle. In addition,
quercetin stimulated the expression of mRNA for
p21(waf1/cip1), which inhibits the activity
of cyclin-dependent kinases, and inhibited the
phosphorylation of histone H1. Furthermore, after
cells had ceased to proliferate, quercetin reduced the
activity of alkaline phosphatase, the level of
expression of mRNA for osteocalcin, the rate of
deposition of Ca(2+), and the formation of mineralized
nodules, all of which are markers of osteoblast
differentiation. These findings indicate that
quercetin inhibits the proliferation, differentiation, and
mineralization of osteoblastic cells.
PMID: 14757127
==========================
==========================
======
J Bone Miner Res. 2000 Nov;15(11):2251-8.
Comment in:
J Bone Miner Res. 2001 May;16(5):970-1.
Rutin inhibits ovariectomy-induced osteopenia in rats.
Horcajada-Molteni MN, Crespy V, Coxam V, Davicco MJ,
Rémésy C, Barlet JP.
Unité Maladies Métaboliques et Micronutriments,
Institut National de la Recherche
Agronomique, Clermont-Theix, France.
Several studies suggest that polyphenols might exert a protective
effect against osteopenia. The present experiment was
conducted to observe the effects of rutin
(quercetin-3-O-glucose rhamnose) on bone metabolism
in ovariectomized (OVX) rats. Thirty 3-month-old Wistar
rats were used. Twenty were OVX while the 10 controls
were sham-operated (SH). Among the 20 OVX, for 90 days
after surgery 10 were fed the same synthetic diet as the
SH or OVX ones, but 0. 25% rutin (OVX + R) was
added. At necropsy, the decrease in uterine weight was
not different in OVX and OVX + R rats. Ovariectomy also
induced a significant decrease in both total and
distal metaphyseal femoral mineral density, which was
prevented by rutin consumption. Moreover, femoral
failure load, which was not different in OVX and
SH rats, was even higher in OVX + R rats than in
OVX or SH rats. In the same way, on day 90,
both urinary deoxypyridinoline (DPD) excretion
(a marker for bone resorption) and calciuria were
higher in OVX rats than in OVX + R or SH rats.
Simultaneously, plasma osteocalcin (OC) concentration
(a marker for osteoblastic activity) was higher in
OVX + R rats than in SH rats. High-performance liquid
chromatography (HPLC) profiles of plasma samples from
OVX + R rats revealed that mean plasma concentration of
active metabolites (quercetin and isorhamnetin) from
rutin was 9.46+/-1 microM, whereas it was undetectable
in SH and OVX rats. These results indicate that rutin
(and/or its metabolites), which appeared devoid of
any uterotrophic activity, inhibits ovariectomy-induced
trabecular bone loss in rats, both by slowing down
resorption and increasing osteoblastic activity.
PMID: 11092407
==========================
==========
Flavonoid quercetin decreases osteoclastic differentiation
induced by RANKL via a mechanism involving NF kappa B and AP-1.
Wattel A, Kamel S, Prouillet C, Petit JP, Lorget F,
Offord E, Brazier M.
Unité de Recherche sur les Mécanismes de la Résorption Osseuse,
Laboratoire de Pharmacie Clinique,
Université de Picardie Jules Verne,
1 rue des Louvels, 80037
Amiens, France.
alice.wattel@sa.u-picardie . fr
Flavonoids are micronutrients widely present in food of
plant origin. They have been attributed pharmacological
properties such as anticancer and prevention of
age-related pathologies. It has been recently
hypothesized that flavonoids increase bone mass
and prevent osteoporosis. However, little is
known about the in vitro effects of flavonoids on
osteoclast activities. We investigated the
effects of quercetin, one of the most commonly
occurring flavonoids, on osteoclast differentiation
which is a critical determinant step of in vivo bone
resorption. Two in vitro models of osteoclast
differentiation were used in this study: a murine one,
involving the culture of RAW 264.7 cells in presence of
receptor activator of NF kappa B ligand (RANKL),
and a human model consisting of differentiating
peripheral blood monocytic cells (PBMC) isolated
from peripheral blood in presence of RANKL and
macrophage-colony stimulating factor (M-CSF).
Osteoclastogenesis was assessed by osteoclast-like
number, tartrate resistant acid phosphatase (TRAP)
activity, and bone resorbing activity. We showed that
quercetin (0.1-10 microM) decreased osteoclastogenesis
in a dose dependent manner in both models with
significant effects observed at low concentrations,
from 1 to 5 microM. The IC(50) value was about
1 microM. Analysis of protein-DNA
interaction by electrophoretic mobility shift assay
(EMSA) performed on RAW cells showed that a pre-treatment
with quercetin inhibited RANKL-induced nuclear factor
kB (NF kappa B) and activator protein 1 (AP-1) activation.
NF kappa B and AP-1 are transcription factors highly
involved in osteoclastic differentiation and
their inhibition could play an important role in
the decrease of osteoclastogenesis observed in
the presence of quercetin. In conclusion, the
present results demonstrate for the first time
that quercetin, a flavonoid characterized by
antioxidant activities, is a potent inhibitor
of in vitro osteoclastic differentiation, via a
mechanism involving NF kappa B and AP-1.
Copyright 2004 Wiley-Liss, Inc.
PMID: 15108355
==========================
======
Biochem Pharmacol. 2004 Apr 1;67(7):1307-13.
Stimulatory effect of naturally occurring flavonols
quercetin and kaempferol on alkaline phosphatase
activity in MG-63 human osteoblasts through ERK and estrogen
receptor pathway.
Prouillet C, Mazière JC, Mazière C, Wattel A,
Brazier M, Kamel S.
Laboratoire de Biochimie,
JE 23 29, CHRU d'Amiens,
Hôpital Nord, 80054 Amiens
Cedex 01, France.
Many plant-derived substances have estrogenic activities.
Due to their ability to bind the estrogen receptor (ER),
these compounds have the potential to counteract
the deleterious effects of estrogen deficiency
on bone. In this study, we investigated the
in vitro effect of two widespread flavonols,
quercetin and kaempferol, on alkaline phosphatase (ALP)
activity in MG-63 cultured human osteoblasts.
We found that both flavonols significantly
increased ALP activity. This effect was markedly
reduced by PD 98059, an inhibitor of the extracellular
regulated kinase (ERK) pathway, and by ICI 182780, an
antagonist of ERs. Western blot studies confirmed that
ERK is rapidly activated in cells treated by both
flavonols. Finally, ICI 182780 markedly inhibits
the flavonol-induced ERK activation. The data presented
in this study support the conclusion that, in
MG-63 osteoblasts (i) the increase in ALP activity
by flavonols involves a rapid stimulation of ERK
activation but also involves the ER, and that (ii) the
activation of ERK by flavonols occurs most likely
downstream of the ERs activation. Taken together,
these results suggest that flavonols derivatives as
quercetin and kaempferol can stimulate osteoblastic
activity. Such compounds may represent
new pharmacological tools for the treatment of osteoporosis.
PMID: 15013846
A collection of abstracts that suggest
rutin, quercetin, and other flavonoids help
preserve bone.
Here in the States it is possible to buy both
bulk quercetin and rutin powders as well as
tablets. Quercetin powder is very light
and is a little hard to mix into water.
Some foods are pretty good sources.
The USDA has some downloads on
food sources for flavonoids and related
substances.
==========================
===
Zhong Yao Cai. 2006 May;29(5):467-70.
[Comparative study on effects of rutin and quercetin
on metabolism in osteoblast
cells]
[Article in Chinese]
Yang YJ, Yang ZL, Wang DC, Xiao XC, Li P.
Department of Traditional Chinese Medicine,
China Pharmaceutical University,
Nanjing 210038, China.
yangyajunl@163 . com
OBJECTIVE:
To investigate the mechanism of rutin and its aglycone
(quercetin) in preventing and treating osteoporosis and
their effects on stimulating bone
formation were studied comparatively in osteoblast cells.
METHODS:
The effects of rutin and quercetin on the proliferation,
differentiation and mineralization were studied respectively
by using tetrazolium (MTT), alkaline phosphatase (ALP) and
mineralized nodules assays in newborn rat calvarial
osteoblast (ROB) cells.
RESULTS:
Quercetin showed significant stimulatory effects on
proliferation and mineralization in ROB cells, so it could
promote bone formation. However, rutin could not improve
stimulation of bone formation because it increased
proliferation and inhibited differentation and had
no remarkable influence on ROB cells in vitro.
CONCLUSION:
Rutin showed less activity than quercetin on bone
formation in ROB cells in vitro. Therefore the
mechanism that some foods and traditional Chinese medicine
containing rutin can prevent and treat osteoporosis
is that its metaboite in vivo can promote bone formation
in ROB cells.
PMID: 16981462
==========================
=====================
Eur J Pharmacol. 2004 Feb 6;485(1-3):89-96.
Quercetin, a flavonoid, inhibits the proliferation,
differentiation, and mineralization of osteoblasts in vitro.
Notoya M, Tsukamoto Y, Nishimura H, Woo JT, Nagai K,
Lee IS, Hagiwara H.
Department of Biological Sciences,
Tokyo Institute of Technology, 4259
Nagatsuta-cho, Midori-ku,
Yokohama 226-8501, Japan.
It is possible that the flavonoids that are found in
many foods might have a protective effect against osteoclastic
activity. However, little information is available about
the effects of flavonoids on osteoblastogenesis.
Therefore, we investigated the effects of quercetin,
a flavonoid, on the metabolism of rat
calvarial osteoblast-like cells (ROB cells) in culture.
The proliferation of cells was markedly inhibited upon
exposure of cells to quercetin at 5 x 10(-6) to
1 x 10(-5) M. Quercetin at 1 x 10(-5) M did not
induce apoptosis in ROB cells but arrested cells
at the G1 phase of the cell cycle. In addition,
quercetin stimulated the expression of mRNA for
p21(waf1/cip1), which inhibits the activity
of cyclin-dependent kinases, and inhibited the
phosphorylation of histone H1. Furthermore, after
cells had ceased to proliferate, quercetin reduced the
activity of alkaline phosphatase, the level of
expression of mRNA for osteocalcin, the rate of
deposition of Ca(2+), and the formation of mineralized
nodules, all of which are markers of osteoblast
differentiation. These findings indicate that
quercetin inhibits the proliferation, differentiation, and
mineralization of osteoblastic cells.
PMID: 14757127
==========================
==========================
======
J Bone Miner Res. 2000 Nov;15(11):2251-8.
Comment in:
J Bone Miner Res. 2001 May;16(5):970-1.
Rutin inhibits ovariectomy-induced osteopenia in rats.
Horcajada-Molteni MN, Crespy V, Coxam V, Davicco MJ,
Rémésy C, Barlet JP.
Unité Maladies Métaboliques et Micronutriments,
Institut National de la Recherche
Agronomique, Clermont-Theix, France.
Several studies suggest that polyphenols might exert a protective
effect against osteopenia. The present experiment was
conducted to observe the effects of rutin
(quercetin-3-O-glucose rhamnose) on bone metabolism
in ovariectomized (OVX) rats. Thirty 3-month-old Wistar
rats were used. Twenty were OVX while the 10 controls
were sham-operated (SH). Among the 20 OVX, for 90 days
after surgery 10 were fed the same synthetic diet as the
SH or OVX ones, but 0. 25% rutin (OVX + R) was
added. At necropsy, the decrease in uterine weight was
not different in OVX and OVX + R rats. Ovariectomy also
induced a significant decrease in both total and
distal metaphyseal femoral mineral density, which was
prevented by rutin consumption. Moreover, femoral
failure load, which was not different in OVX and
SH rats, was even higher in OVX + R rats than in
OVX or SH rats. In the same way, on day 90,
both urinary deoxypyridinoline (DPD) excretion
(a marker for bone resorption) and calciuria were
higher in OVX rats than in OVX + R or SH rats.
Simultaneously, plasma osteocalcin (OC) concentration
(a marker for osteoblastic activity) was higher in
OVX + R rats than in SH rats. High-performance liquid
chromatography (HPLC) profiles of plasma samples from
OVX + R rats revealed that mean plasma concentration of
active metabolites (quercetin and isorhamnetin) from
rutin was 9.46+/-1 microM, whereas it was undetectable
in SH and OVX rats. These results indicate that rutin
(and/or its metabolites), which appeared devoid of
any uterotrophic activity, inhibits ovariectomy-induced
trabecular bone loss in rats, both by slowing down
resorption and increasing osteoblastic activity.
PMID: 11092407
==========================
==========
Flavonoid quercetin decreases osteoclastic differentiation
induced by RANKL via a mechanism involving NF kappa B and AP-1.
Wattel A, Kamel S, Prouillet C, Petit JP, Lorget F,
Offord E, Brazier M.
Unité de Recherche sur les Mécanismes de la Résorption Osseuse,
Laboratoire de Pharmacie Clinique,
Université de Picardie Jules Verne,
1 rue des Louvels, 80037
Amiens, France.
alice.wattel@sa.u-picardie . fr
Flavonoids are micronutrients widely present in food of
plant origin. They have been attributed pharmacological
properties such as anticancer and prevention of
age-related pathologies. It has been recently
hypothesized that flavonoids increase bone mass
and prevent osteoporosis. However, little is
known about the in vitro effects of flavonoids on
osteoclast activities. We investigated the
effects of quercetin, one of the most commonly
occurring flavonoids, on osteoclast differentiation
which is a critical determinant step of in vivo bone
resorption. Two in vitro models of osteoclast
differentiation were used in this study: a murine one,
involving the culture of RAW 264.7 cells in presence of
receptor activator of NF kappa B ligand (RANKL),
and a human model consisting of differentiating
peripheral blood monocytic cells (PBMC) isolated
from peripheral blood in presence of RANKL and
macrophage-colony stimulating factor (M-CSF).
Osteoclastogenesis was assessed by osteoclast-like
number, tartrate resistant acid phosphatase (TRAP)
activity, and bone resorbing activity. We showed that
quercetin (0.1-10 microM) decreased osteoclastogenesis
in a dose dependent manner in both models with
significant effects observed at low concentrations,
from 1 to 5 microM. The IC(50) value was about
1 microM. Analysis of protein-DNA
interaction by electrophoretic mobility shift assay
(EMSA) performed on RAW cells showed that a pre-treatment
with quercetin inhibited RANKL-induced nuclear factor
kB (NF kappa B) and activator protein 1 (AP-1) activation.
NF kappa B and AP-1 are transcription factors highly
involved in osteoclastic differentiation and
their inhibition could play an important role in
the decrease of osteoclastogenesis observed in
the presence of quercetin. In conclusion, the
present results demonstrate for the first time
that quercetin, a flavonoid characterized by
antioxidant activities, is a potent inhibitor
of in vitro osteoclastic differentiation, via a
mechanism involving NF kappa B and AP-1.
Copyright 2004 Wiley-Liss, Inc.
PMID: 15108355
==========================
======
Biochem Pharmacol. 2004 Apr 1;67(7):1307-13.
Stimulatory effect of naturally occurring flavonols
quercetin and kaempferol on alkaline phosphatase
activity in MG-63 human osteoblasts through ERK and estrogen
receptor pathway.
Prouillet C, Mazière JC, Mazière C, Wattel A,
Brazier M, Kamel S.
Laboratoire de Biochimie,
JE 23 29, CHRU d'Amiens,
Hôpital Nord, 80054 Amiens
Cedex 01, France.
Many plant-derived substances have estrogenic activities.
Due to their ability to bind the estrogen receptor (ER),
these compounds have the potential to counteract
the deleterious effects of estrogen deficiency
on bone. In this study, we investigated the
in vitro effect of two widespread flavonols,
quercetin and kaempferol, on alkaline phosphatase (ALP)
activity in MG-63 cultured human osteoblasts.
We found that both flavonols significantly
increased ALP activity. This effect was markedly
reduced by PD 98059, an inhibitor of the extracellular
regulated kinase (ERK) pathway, and by ICI 182780, an
antagonist of ERs. Western blot studies confirmed that
ERK is rapidly activated in cells treated by both
flavonols. Finally, ICI 182780 markedly inhibits
the flavonol-induced ERK activation. The data presented
in this study support the conclusion that, in
MG-63 osteoblasts (i) the increase in ALP activity
by flavonols involves a rapid stimulation of ERK
activation but also involves the ER, and that (ii) the
activation of ERK by flavonols occurs most likely
downstream of the ERs activation. Taken together,
these results suggest that flavonols derivatives as
quercetin and kaempferol can stimulate osteoblastic
activity. Such compounds may represent
new pharmacological tools for the treatment of osteoporosis.
PMID: 15013846
Re: calci-chew
PS>Is anyone out there taking a calcium+D3 supplement that does not have
>aspartame in it or other sugar substitutes? I had started taking the calci
>chew but the flyer mentions aspartame as ingredient.
I'm not trying to sell anything, but I am taking Shaklee's Osteomatrix,
completely natural and formulated to be taken in 4 tablets spread
through the day. I've used this company for over 30 years.
I haven't been good about taking calcium though. I had a bone scan
done a couple of weeks ago and found that I had lost about 14 percent
in my spine. The doctor put me on fosomax. I've always exercised -
walking, jogging, etc so my pelvis and legs are good shape, but I've
never done any back exercises. I'm 50.
AmyW
* 1st 2.00 #8266 *
PS>Is anyone out there taking a calcium+D3 supplement that does not have
>aspartame in it or other sugar substitutes? I had started taking the calci
>chew but the flyer mentions aspartame as ingredient.
I'm not trying to sell anything, but I am taking Shaklee's Osteomatrix,
completely natural and formulated to be taken in 4 tablets spread
through the day. I've used this company for over 30 years.
I haven't been good about taking calcium though. I had a bone scan
done a couple of weeks ago and found that I had lost about 14 percent
in my spine. The doctor put me on fosomax. I've always exercised -
walking, jogging, etc so my pelvis and legs are good shape, but I've
never done any back exercises. I'm 50.
AmyW
* 1st 2.00 #8266 *
Re: calci-chew
Hi, Pam,
If you are looking for calcium supplement, it might be worth thinking
of one with the right proporotion of the other vitamins and
minerals you need to have the calcium get used by your body to build
bone. ie, Vitamin D, K, trace minerals boron, silica ....or plan to
add foods with those things to your diet.
Most calicum supplements state on the pack what they contain by way of
trace minerals, vitamins etc.
You can check the gov. web site PU MED for articles that have been
published that shown the effectivenss of various calcium supplements
by brand. (One that advertises widely in my area of the country and
claims 'scientific studies' in their ads - but never cite which
studies......that brand shows up in last or next to last rank in any
of the published articles listed on the gov. Pub Med site when it was
compared with other brands of calcium. I guess anyone can claim
anything in an ad....
There is also a page the gives links to some of the science evidence
for various brands of calcium at
* w w w .osteopenia3 . com /Calcium-supplements.html
Kate
dmOn Dec 25, 9:24 am, "perkens" <perk...@home.nl> wrote:
> Hi Members, I am new to the group.Recently found out I have osteoporosis,
> age 52 female, menopause at age 40 .
>
> I was prescribed actonel and calci chew but am trying to find homeopathic
> alternatives as I don't like the sound of either one.
> Is anyone out there taking a calcium+D3 supplement that does not have
> aspartame in it or other sugar substitutes? I had started taking the calci
> chew but the flyer mentions aspartame as ingredient.
>
> Thanks, Pam
Hi, Pam,
If you are looking for calcium supplement, it might be worth thinking
of one with the right proporotion of the other vitamins and
minerals you need to have the calcium get used by your body to build
bone. ie, Vitamin D, K, trace minerals boron, silica ....or plan to
add foods with those things to your diet.
Most calicum supplements state on the pack what they contain by way of
trace minerals, vitamins etc.
You can check the gov. web site PU MED for articles that have been
published that shown the effectivenss of various calcium supplements
by brand. (One that advertises widely in my area of the country and
claims 'scientific studies' in their ads - but never cite which
studies......that brand shows up in last or next to last rank in any
of the published articles listed on the gov. Pub Med site when it was
compared with other brands of calcium. I guess anyone can claim
anything in an ad....
There is also a page the gives links to some of the science evidence
for various brands of calcium at
* w w w .osteopenia3 . com /Calcium-supplements.html
Kate
dmOn Dec 25, 9:24 am, "perkens" <perk...@home.nl> wrote:
> Hi Members, I am new to the group.Recently found out I have osteoporosis,
> age 52 female, menopause at age 40 .
>
> I was prescribed actonel and calci chew but am trying to find homeopathic
> alternatives as I don't like the sound of either one.
> Is anyone out there taking a calcium+D3 supplement that does not have
> aspartame in it or other sugar substitutes? I had started taking the calci
> chew but the flyer mentions aspartame as ingredient.
>
> Thanks, Pam
