Normal birth from cryopreserved embryos after intracytoplasmic sperm injection of frozen semen into

Normal birth from cryopreserved embryos after intracytoplasmic sperm injection of frozen semen into

Normal birth from cryopreserved embryos after intracytoplasmic sperm
injection of frozen semen into vitrified human oocytes

Authors: Gui An Chen a; Xue Yong Cai b; Ying Lian a; Xiao Ying
Zheng a; Jie Qiao a; Xin Na Chen a; Rong Hua Ye a

Affiliations: a Department of Obstetrics & Gynecology, Third
Hospital, Peking University, Peking, China
b Department of Obstetrics & Gynecology, First Hospital, Peking
University, Peking, China

Published in: Human Fertility, Volume 11, Issue 1 2008 , pages 49 -
51

Objective: To describe the birth achieved from frozen embryos after
intracytoplasmic sperm injection (ICSI) of donor sperm into vitrified
oocytes.

Patient: A 25-year-old woman whose husband was azoospermic undergoing
IVF therapy.

Methods: Oocytes collected after ovarian stimulation were vitrified,
thawed, and fertilized by frozen donor sperm.

Results: Nineteen oocytes were vitrified and all survived after
thawing. Thirteen of the 19 oocytes that underwent ICSI with donors
sperm were successfully fertilized. Twelve embryos were cryopreserved
again by conventional slow-freezing protocol because of uterine
bleeding on the day of transfer. Three thawed embryos were
transferred, and a normal male with an infant karyotype of 46,XY was
delivered.

Conclusion: This case report demonstrates effective oocyte
cryopreservation by vitrification.